MSCA PhD fellow Kandeel Shafique invited to present the Biosens4PrecisionMastitis at the EuroScience Open Forum 2022 MSCA Satellite Event


The Marie Skłodowska-Curie Actions Unit, Directorate-General for Education, Youth, Sport and Culture, European Commission, invited MSCA PhD fellow Kandeel Shafique, and her PhD supervisor Beatriz Prieto Simón to attend the EuroScience Open Forum 2022 MSCA Satellite Event on 12 and 13 July in Leiden (Netherlands). The event brought together MSCA fellows, supervisors, coordinators and alumni to debate and contribute to priority topics for the research ecosystem, and to gather, interact and network. The agenda included panel discussions, practical workshops, a poster session, social events and networking opportunities. MSCA PhD fellow Kandeel Shafique’s poster presenting her work on developing novel biosensors for the early diagnosis of bovine mastitis, in the frame of our Biosens4PrecisionMastitis project, was selected to be included in the poster session. She did an excellent job attracting the attention of the event attendees, who asked lots of questions about the suitability of the targeted biomarkers, biosensor performance, etc.

The project’s second publication has been published in an international, peer-reviewed, open-access journal “Pathogens” of MDPI!

This study demonstrates a comprehensive report on inflammatory and regulatory cytokines in cows affected by naturally occurring subclinical and clinical mastitis. In naturally occurring mastitis, knowledge, at the cellular and molecular level, of the host immune response and during the infection is fundamental to the early and timely diagnosis, and in perspective pathogenesis-based treatment. Immune response in mastitis is characterized by continuation or resolution of initial inflammation to protect the udder, reduce transiently the risk of infection while lowering the severity of mastitis.

Second round of milk samples collection completed

Improved experimental protocols as well as foundations for the preliminary panel of biomarkers for early mastitis detection – those were the major highlights of the second round of 6-day-long field tests in Biosens4PrecisionMastitis that ended on October 18, 2021 in Urbanki (Northern Poland).

This was another important experimental step in our project, which brings us closer to fulfilling our research vision: distinguishing between mastitis infected and healthy cows at the early stages of the disease.

This Autumn we collected milk samples from the same dairy farm we visited in Spring. Based on the insights and experiences we collected previously, we were able to improve our experimental protocols and collect valuable knowledge base for the next steps of our project

– explains Lukasz Gontar from Research and Innovation Centre Pro-Akademia, one of the partners in Biosens4PrecisionMastitis project, who coordinated the field experiments, microbiological studies, Somatic Cell Count analyses, isolation of total RNA, and preparation of milk sediments.

Similar to the previous sample collection round, the research has showed that new methods for early mastitis detection are urgently needed. Based on the collected samples of milk of dairy cows with subclinical and clinical mastitis, Biosens4PrecisionMastitis partners in Hungary (Seqomics) and Latvia (RSU) are now preparing miRNA-specific libraries as well as identifying inflammatory and regulatory cytokines, which can serve as biomarkers for early mastitis detection. The first scientific publication on our joint results is expected soon – please stay tuned!

First round of milk samples collection completed

2021-06-21: First round of milk samples collection completed

With a total of 600 milk samples collected from 35 cows, the first round of field tests in Biosens4PrecisionMastitis ended on June 21, 2021 in Urbanki (Northern Poland). This was an important experimental step in our project, which will allow for the identification of miRNAs and key cytokines/antimicrobial peptides with a potential to distinguish between infected and healthy cows by our novel biosensors.


Biosens4PrecisionMastitis innovation relies on non-invasive and stress-free milk biopsies. This is why milk is our focal point. Following clinical observations of animals and investigations of somatic cell count in milk, we have identified cows with clinical mastitis, subclinical mastitis and healthy cows

– explains Lukasz Gontar from Research and Innovation Centre Pro-Akademia, one of the partners in Biosens4PrecisionMastitis project, who coordinated the field experiments and the first step of microbiological research.

The collected milk samples have showed that mastitis can be caused by many different pathogens, while also different biomarkers could be useful for mastitis detection. We are looking forward to sharing the details on our first results with you very soon!


ICRAD initial grant holders’ meeting

On May 27th, the ICRAD initial grant holders’ meeting took place on-line. All ICRAD funded projects were presented by the Project Coordinators. It was a very interesting journey to better understand animal diseases, technology platforms to produce vaccines, and novel detection technologies. Our project closed the seminar presenting new sensing technology to achieve prompt and efficient diagnosis of bovine mastitis.

Kick-off meeting

We are excited to announce we had our kick-off meeting on the 7th April 2021!

On the 7th April 2021, the Biosens4PrecisionMastitis project had its formal project kick-off meeting. The event provided room for in-depth discussions, knowledge sharing, and opportunites to improve our planned scientific collaborations.

Thekick-offmeeting was the first official event in the project that allowed for the consortium to meet, albeit virtually. Short presentations were given by each partner, which provided a clear overview of our joint objectives towards early and cost-effective mastitis detection across Europe.