This study demonstrates a comprehensive report on inflammatory and regulatory cytokines in cows affected by naturally occurring subclinical and clinical mastitis. In naturally occurring mastitis, knowledge, at the cellular and molecular level, of the host immune response and during the infection is fundamental to the early and timely diagnosis, and in perspective pathogenesis-based treatment. Immune response in mastitis is characterized by continuation or resolution of initial inflammation to protect the udder, reduce transiently the risk of infection while lowering the severity of mastitis.
Improved experimental protocols as well as foundations for the preliminary panel of biomarkers for early mastitis detection – those were the major highlights of the second round of 6-day-long field tests in Biosens4PrecisionMastitis that ended on October 18, 2021 in Urbanki (Northern Poland).
This was another important experimental step in our project, which brings us closer to fulfilling our research vision: distinguishing between mastitis infected and healthy cows at the early stages of the disease.
This Autumn we collected milk samples from the same dairy farm we visited in Spring. Based on the insights and experiences we collected previously, we were able to improve our experimental protocols and collect valuable knowledge base for the next steps of our project
– explains Lukasz Gontar from Research and Innovation Centre Pro-Akademia, one of the partners in Biosens4PrecisionMastitis project, who coordinated the field experiments, microbiological studies, Somatic Cell Count analyses, isolation of total RNA, and preparation of milk sediments.
Similar to the previous sample collection round, the research has showed that new methods for early mastitis detection are urgently needed. Based on the collected samples of milk of dairy cows with subclinical and clinical mastitis, Biosens4PrecisionMastitis partners in Hungary (Seqomics) and Latvia (RSU) are now preparing miRNA-specific libraries as well as identifying inflammatory and regulatory cytokines, which can serve as biomarkers for early mastitis detection. The first scientific publication on our joint results is expected soon – please stay tuned!
We are proud to announce that we have just uploaded to our YouTube channel an engaging video that describes the aims of our Biosens4PrecisionMastitis project, providing details of the technology we are developing, and discussing the expected project impact. Hope you enjoy it and find the story inspiring and entertaining!
The aims, background and consortium of Biosens4PrecisionMastitis project are presented at URV website.
The Biosens4PrecisionMastitis project aims are included in the 2nd Issue of the ICRAD Newsletter.
Further information at: https://www.icrad.eu/wp-content/uploads/2021/07/ICRAD_Newsletter2.pdf
2021-06-21: First round of milk samples collection completed
With a total of 600 milk samples collected from 35 cows, the first round of field tests in Biosens4PrecisionMastitis ended on June 21, 2021 in Urbanki (Northern Poland). This was an important experimental step in our project, which will allow for the identification of miRNAs and key cytokines/antimicrobial peptides with a potential to distinguish between infected and healthy cows by our novel biosensors.
Biosens4PrecisionMastitis innovation relies on non-invasive and stress-free milk biopsies. This is why milk is our focal point. Following clinical observations of animals and investigations of somatic cell count in milk, we have identified cows with clinical mastitis, subclinical mastitis and healthy cows
– explains Lukasz Gontar from Research and Innovation Centre Pro-Akademia, one of the partners in Biosens4PrecisionMastitis project, who coordinated the field experiments and the first step of microbiological research.
The collected milk samples have showed that mastitis can be caused by many different pathogens, while also different biomarkers could be useful for mastitis detection. We are looking forward to sharing the details on our first results with you very soon!
The first publication within this project, the literature review article states a broad overview of the factors and their role in the development of inflammation in bovine mastitis.
On May 27th, the ICRAD initial grant holders’ meeting took place on-line. All ICRAD funded projects were presented by the Project Coordinators. It was a very interesting journey to better understand animal diseases, technology platforms to produce vaccines, and novel detection technologies. Our project closed the seminar presenting new sensing technology to achieve prompt and efficient diagnosis of bovine mastitis.
We are excited to announce we had our kick-off meeting on the 7th April 2021!
On the 7th April 2021, the Biosens4PrecisionMastitis project had its formal project kick-off meeting. The event provided room for in-depth discussions, knowledge sharing, and opportunites to improve our planned scientific collaborations.
Thekick-offmeeting was the first official event in the project that allowed for the consortium to meet, albeit virtually. Short presentations were given by each partner, which provided a clear overview of our joint objectives towards early and cost-effective mastitis detection across Europe.